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The simulated influences of electromagnetic radiation of natural and technogenic origin on tumor cell line hep-2 in vitro

Keywords:

S.N. Darovskikh - Dr.Sc. (Eng.), Professor, Head of Department of Information and Communication Technologies, South Ural State University (National Research University), Chelyabinsk E-mail: darovskih.s@mail.ru I.I. Dolgushin - Corresponding Member of the RAS, Dr.Sc. (Med.), Professor, Rector, Head of Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, South Ural State Medical University Ministry of Health of the Russian Federation, Chelyabinsk E-mail: dol-ii@mail.ru Yu.S. Shishkova - Dr.Sc. (Med.), Professor, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, South Ural State Medical University Ministry of Health of the Russian Federation, Chelyabinsk E-mail: shishkova_yulia@mail.ru A.B. Semenova - Ph.D. (Med.), Head of Laboratory and Diagnostic Service, Chelyabinsk Regional Clinical Oncology Center E-mail: asemenova81@mail.ru E.L. Kazachkov - Dr.Sc. (Med.), Professor, Head of Department of Pathological Anatomy, South Ural State Medical University Ministry of Health of the Russian Federation, Chelyabinsk E-mail: doctorkel@narod.ru A.V. Vazhenin - Corresponding Member of the RAS, Dr.Sc. (Med.), Professor, Honored Doctor of Russia, Chief Physician, Chelyabinsk Regional Clinical Oncology Center E-mail: vav222@mail.ru N.V. Vdovina - Ph.D. (Eng), Senior Lecturer, Department of Information and Communication Technologies, South Ural State University (National Research University), Chelyabinsk E-mail: nadzh@yandex.ru G.G. Chirkova - Head of Virologic Laboratory, Center for Hygiene and Epidemiology in Chelyabinsk region, Chelyabinsk E-mail: viruslab@chel.surnet.ru


The results of experimental studies, aimed to assess the influence of simulated low intensity (no more than 100 µW/cm2) elec-tromagnetic radiation (EMR) of natural and technogenic origin on the transplanted cell lines of epidermoid carcinoma of the larynx, HEp-2 clone, in conditions of their co-incubation with neutrophilic granulocytes, are presented. It is established that a direct short (16 minutes) exposure to the tumor cells of HEp-2 clone by simulated solar radiation of the microwave frequency range (4.0-4.3) GHz results in their complete irreversible destruction. There was a similar experiment using EMR of technogenic origin (the carrier frequency is 4.1 GHz, the intensity - no more than 100 µW/cm2), where the death of the most cells was determined. However, the cancer cells, which retained their viability, after 24 hours of incubation, restored their population comparable to the control indicators. The interaction of tumor cells of HEp-2 clone and neutrophils in the experiment with the EMR mentioned above leads to the formation of the extracellular DNA networks by neutrophilic granulocytes, which entwine the tumor cells, bringing them closer to each other. After 24 hours of cultivation a significant increase in the number of tumor cells, presence of conglomerates, complexes and simplasts was registered. Apparently, the revealed effect is due to the fact that neutrophil extracellular DNA has a shielding effect, which preserve the viability of tumor cells in conditions of electromagnetic radiation of different origin and promotes their proliferation.
References:

 

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