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Amperometric biosensor analyzer for express-determination of biochemical oxygen demand

Keywords:

V.A. Arlyapov - Ph.D. (Chem.), Associate Professor, Department of Biochemistry, Tula State University E-mail: v.a.arlyapov@gmail.com P.V. Mel’nikov - Ph.D. (Phys.-Math.), Associate Professor, Department of Physical Chemistry, Moscow Technological University E-mail: melnikovsoft@mail.ru N.Yu. Yudina - Assistant, Department of Chemistry, Tula State University E-mail: tysia21-05-90@mail.ru N.K. Zaitsev - Dr.Sc. (Chem.), Associate Professor, Head of Department of Energy Technologies, Plants and Systems, Moscow Technological University E-mail: nk_zaytsev@mail.ru V.A. Alferov - Ph.D. (Chem.), Associate Professor, Head of Department of Chemistry, Tula State University E-mail: chem@tsu.tula.ru A.N. Reshetilov - Dr.Sc. (Chem.), Head of Laboratory, Tula State University; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the RAS, Pushchino, Russia


Biosensor analyzer was developed for express-determination of biochemical oxygen demand (BOD) This biosensor analyzer is–based on an amperometric oxygen electrode and yeast cells. An analyzer for biochemical oxygen demand consists of primary and measuring transducers (MT). The measuring transducer is a microprocessor which provides representation of measurements in graphic form on liquid crystal display as well as offers multi nesting level and various scenarios of work. This analyzer can be used as a stand alone sensor for measurement process or used with special software to collect and store data. This analyzer is based on yeast cells of the genus Debaryomyces. To form the receptor element of a biosensor, yeast cells were immobilized in polyvinyl alcohol modified with N-vinylpirrolidone. With the use of the fabricated analyzer the concentration range of the determined BOD without dilution of a probe was 0,16-30,0 mg О2/dm3. The fabricated analyzer was tested. A correlation between the results obtained and conventional data is high (R = 0,9999). This evidence supports the possibility of using the developed biosensor analyzer as a pilot prototype model for series production.
References:

 

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