Radiotekhnika
Publishing house Radiotekhnika

"Publishing house Radiotekhnika":
scientific and technical literature.
Books and journals of publishing houses: IPRZHR, RS-PRESS, SCIENCE-PRESS


Тел.: +7 (495) 625-9241

 

Investigation of oxidative status in patients with systemic lupus erythematosus by luminescent methods

Keywords:

E.V. Smirnova - Post-graduate Student, Department of Internal Diseases, Faculty of Basic Medicine, Lo-monosov Moscow State University
E-mail: elena.smirnova881@gmail.com
M.M. Sozarukova -Post-graduate Student, Department of Medical Biophysics, Faculty of Basic Medicine, Lomonosov Moscow State University
E-mail: s_madinam@bk.ru
A.M. Polimova - Ph.D. (Biol.), Research Scientist, Federal Research Center “Crystallography and Photon-ics” of the Russian Academy of Sciences (Moscow)
E-mail: corason27@mail.ru
T.N. Krasnova - Ph.D. (Med.), Associate Professor, Department of Internal Diseases, Faculty of Basic Med-icine, Lomonosov Moscow State University
E-mail: krasnovamgu@yandex.ru
Е.V. Proskurina - Ph.D. (Chem.), Associate Professor, Department of Medical Biophysics, Faculty of Basic Medicine, Lomonosov Moscow State University
E-mail: proskurnina@gmail.com


Systemic lupus erythematosus (SLE) is an autoimmune disease, whose molecular mechanisms remain unclear. It is consi-dered that oxidative stress plays a significant role in its pathogenesis. Excessive production of reactive oxygen species (ROS) promotes abnormal activation of apoptosis and impaired clearance of apoptotic bodies, that lead to formation of epitopes, stimulating autoantibodies generation. With this respect, oxidative stress markers appear to be potential indicators for as-sessment of disease severity and treatment effectiveness, their investigation might be valuable for search of new treatment targets. Therefore, the new methods were proposed for rapid evaluation of oxidative stress in blood plasma of patients with systemic lupus erythematosus (SLE): plasma antioxidant activity (AOA) assessment using kinetic luminol-activated chemi-luminescence and assessment of albumin oxidation state using spectrofluorometry. It was shown, that tryptophan fluores-cence of globulin free blood plasma reflects the total level of CIC and albumin. Blood plasma of 53 patients with SLE was analyzed. It was found, that plasma AOA in patients with SLE exceeds such in controls, but significant differences between patient groups were not shown. Therefore, the necessity of antioxidant therapy appears to be doubtful. It was found that the fraction of oxidized albumin in patients with SLE does not differ significantly from this parameter of healthy controls. Tryp-tophan fluorescence of globulin free plasma is reliably higher in the group with internal organ involvement, comparing to the group without it; in the group with active renal disease, comparing to the group without it, as well as in the group re-ceiving active immunosuppressive therapy including biologic therapy or therapy with high-dose steroids and cyclophos-phamide, comparing to the group receiving maintenance therapy. The correlation of this parameter with concentration of anti-DNA antibodies was shown. Therefore, it can be concluded, that tryptophan fluorescence may be used in estimation of CIC level in SLE patients’ plasma. Further investigations with larger patients group appear to be promising to obtain more reliable results. It might allow to compare levels of oxidative stress in patients with different activity and organ damage, and at various stages of treatment and follow-up to develop new diagnostic and prognostic criteria.

References:
  1. Graham K.L., Utz P.J. Sources of autoantigens in systemic lupus erythematosus // Current Opinion in Rheumatology. 2005. V. 17. № 5. P. 513–517.
  2. Munoz L.E., van Bavel C., Franz S., Berden J., Herrmann M., van der Vlag J. Apoptosis in the pathogenesis of systemic lupus erythematosus // Lupus. 2008. V. 17. № 5. P. 371–375.
  3. Ahsan H., Ali A., Ali R. Oxygen free radicals and systemic autoimmunity // Clinical and Experimental Immunolgy. 2003. V. 131. № 3. P. 398–404.
  4. Kim-Howard X., Sun C., Molineros J.E., Maiti A.K., Chandru H., Adler A., Wiley G.B., Kaufman K.M., Kottyan L., Guthridge J.M., Rasmussen A., Kelly J., Sánchez E., Raj P., Li Q.Z., Bang S.Y., Lee H.S., Kim T.H., Kang Y.M., Suh C.H., Chung W.T., Park Y.B., Choe J.Y., Shim S.C., Lee S.S., Han B.G., Olsen N.J., Karp D.R., Moser K., Pons-Estel B.A., Wakeland E.K., James J.A., Harley J.B., Bae S.C., Gaffney P.M., Alarcón-Riquelme M. Allelic heterogeneity in NCF2 associated with systemic lupus erythematosus (SLE) susceptibility across four ethnic populations // Human Molecular Genetics. 2014. V. 23. № 6. P. 1656–1668.
  5. Warchol T., Lianeri M., Wudarski M., Łacki JK., Jagodziński P.P. Catalase -262C > T polymorphism in systemic lupus erythematosus in Poland // Rheumatology International. 2008. V. 28. № 10. P. 1035–1039.
  6. Kiyohara C., Washio M., Horiuchi T., Asami T., Ide S., Atsumi T., Kobashi G., Takahashi H., Tada Y. Risk modification by CYP1A1 and GSTM1 polymorphisms in the association of cigarette smoking and systemic lupus erythematosus in a Japanese population // Scandinavian Journal of Rheumatology. 2012. V. 41. № 2. P.103–109.
  7. Ollier W., Davies E., Snowden N., Alldersea J., Fryer A., Jones P., Strange R. Association of homozygosity for glutathione-S-transferase GSTM1 null alleles with the Ro+/La- autoantibody profile in patients with systemic lupus erythematosus // Arthritis and Rheumatism. 1996. V. 39. № 10. P. 1763–1764.
  8. Mansour R.B., Lassoued S., Gargouri B., El Gaïd A., Attia H., Fakhfakh F. Increased levels of autoantibodies against catalase and superoxide dismutase associated with oxidative stress in patients with rheumatoid arthritis and systemic lupus erythematosus // Scandinavian Journal of Rheumatology. 2008. V. 37. № 2. P. 103–108.
  9. Ben Mansour R., Lassoued S., Elgaied A., Haddouk S., Marzouk S., Bahloul Z., Masmoudi H., Attia H., Aïfa M.S., Fakhfakh F. Enhanced reactivity to malondialdehyde-modified proteins by systemic lupus erythematosus autoantibodies // Scandinavian Journal of Rheumatology. 2010. V. 39. № 3. P. 247–253.
  10. Jovanovic V., Abdul Aziz N., Lim Y.T., Ng Ai Poh A., Jin Hui Chan S., Ho Xin Pei E., Lew F.C., Shui G., Jenner A.M., Bowen L., McKinney E.F., Lyons P.A., Kemeny M.D., Smith K.G., Wenk M.R., Macary P.A. Lipid anti-lipid antibody responses correlate with disease activity in systemic lupus erythematosus // PLoS One. 2013. 8:e55639.
  11. Shah D., Kiran R., Wanchu A., Bhatnagar A. Oxidative stress in systemic lupus erythematosus: relationship to Th1 cytokine and disease activity // Immunology Letters. 2010. V. 129. № 1. P. 7–12.
  12. Frostegard J., Svenungsson E., Wu R., Gunnarsson I., Lundberg I.E., Klareskog L., Hörkkö S., Witztum J.L. Lipid peroxidation is enhanced in patients with systemic lupus erythematosus and is associated with arterial and renal disease manifestations // Arthritis and Rheumatism. 2005. V. 52. № 1. P. 192–200.
  13. Tewthanom K., Janwityanuchit S., Totemchockchyakarn K., Panomvana D. Correlation of lipid peroxidation and glutathione levels with severity of systemic lupus erythematosus: a pilot study from single center // Journal of Pharmacy and Pharmaceutical Sciences. 2008. V. 11. № 3. P. 30–34.
  14. Lopez L.R., Simpson D.F., Hurley B.L., Matsuura E. OxLDL/beta2GPI complexes and autoantibodies in patients with systemic lupus erythematosus, systemic sclerosis, and antiphospholipid syndrome: pathogenic implications for vascular involvement // Annals of the New York Academy of Sciences. 2005. V. 1051. P. 313–322.
  15. Sozarukova M.M., Polimova A.M., Proskurnina E.V., Vladimirov Ju.A. Izmenenija v kinetike hemiljuminescencii plazmy kak mera sistemnogo okislitel'nogo stressa v organizme cheloveka // Biofizika. 2016. T. 61. S. 337–344.
  16. Sozarukova M.M., Proskurnina E.V., Vladimirov Ju.A. Syvorotochnyj al'bumin kak istochnik i mishen' svobodnyh radikalov v patologii // Vestnik RGMU. 2016. № 1. S. 61–67.
  17. Medicinskie laboratornye tehnologii. Ruko¬vodstvo po klinicheskoj laboratornoj diagnostike / Pod red. prof. A.I. Karpishhenko. M.: Gjeotar-Media. 2013.
  18. Gergely P.J., Grossman C., Niland B., Puskas F., Neupane H., Allam F., Banki K., Phillips P.E., Perl A. Mitochondrial hyperpolarization and ATP depletion in patients with systemic lupus erythematosus // Arthritis and Rheumatism. 2002. V. 46. № 1. P. 175–190.
  19. Gergely P.J., Niland B., Gonchoroff N., Pullmann R.Jr., Phillips P.E., Perl A. Persistent mitochondrial hyperpolarization, increased reactive oxygen intermediate production, and cytoplasmic alkalinization characterize altered IL-10 signaling in patients with systemic lupus erythematosus // Journal of Immunology. 2002. V. 169. № 2. P. 1092–1101.
  20. Shah D., Aggarwal A., Bhatnagar A., Kiran R., Wanchu A. Association between T lymphocyte sub-sets apoptosis and peripheral blood mononuclear cells oxidative stress in systemic lupus erythematosus // Free Radical Research. 2011. V. 45. № 5. P. 559–567.
  21. Segal B.H., Grimm M.J., Khan A.N., Han W., Blackwell T.S. Regulation of innate immunity by NADPH oxidase // Free Radical Biology and Medicine. 2012. V. 53. № 1. P. 72–80.
  22. Lai Z., Hanczko R., Bonilla E., Caza T.N, Clair B., Bartos A., Miklossy G., Jimah J., Doherty E., Tily H., Francis L., Garcia R., Dawood M., Yu J., Ramos I., Coman I., Faraone S.V, Phillips P.E., Perl A. N-acetylcysteine reduces disease activity by blocking mTOR in T-cells of lupus patients // Arthritis and Rheumatism. 2012. V. 64. № 9. P. 2937–2946.
  23. Watad A., Neumann S.G., Soriano A., Amital H., Shoenfeld Y. Vitamin D and systemic lupus erythemathosus: myth or reality? // The Israel Medical Association Journal. 2016. V. 18. № 3-4. P. 177–182.
  24. Morgan P.E., Sturgess A.D., Davies M.J. Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients // Free Radical Research. 2009. V. 43. № 2. P. 117–127.
  25. Morgan P.E., Sturgess A.D., Hennessy A., Davies M.J. Serum protein oxidation and apolipoprotein CIII levels in people with systemic lupus erythematosus with and without nephritis // Free Radical Research. 2007. V. 41. № 12. P. 1301– 1312.

© Издательство «РАДИОТЕХНИКА», 2004-2017            Тел.: (495) 625-9241                   Designed by [SWAP]Studio