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Investigation of oxidative status in patients with systemic lupus erythematosus by luminescent methods


E.V. Smirnova - Post-graduate Student, Department of Internal Diseases, Faculty of Basic Medicine, Lo-monosov Moscow State University
M.M. Sozarukova -Post-graduate Student, Department of Medical Biophysics, Faculty of Basic Medicine, Lomonosov Moscow State University
A.M. Polimova - Ph.D. (Biol.), Research Scientist, Federal Research Center “Crystallography and Photon-ics” of the Russian Academy of Sciences (Moscow)
T.N. Krasnova - Ph.D. (Med.), Associate Professor, Department of Internal Diseases, Faculty of Basic Med-icine, Lomonosov Moscow State University
Е.V. Proskurina - Ph.D. (Chem.), Associate Professor, Department of Medical Biophysics, Faculty of Basic Medicine, Lomonosov Moscow State University

Systemic lupus erythematosus (SLE) is an autoimmune disease, whose molecular mechanisms remain unclear. It is consi-dered that oxidative stress plays a significant role in its pathogenesis. Excessive production of reactive oxygen species (ROS) promotes abnormal activation of apoptosis and impaired clearance of apoptotic bodies, that lead to formation of epitopes, stimulating autoantibodies generation. With this respect, oxidative stress markers appear to be potential indicators for as-sessment of disease severity and treatment effectiveness, their investigation might be valuable for search of new treatment targets. Therefore, the new methods were proposed for rapid evaluation of oxidative stress in blood plasma of patients with systemic lupus erythematosus (SLE): plasma antioxidant activity (AOA) assessment using kinetic luminol-activated chemi-luminescence and assessment of albumin oxidation state using spectrofluorometry. It was shown, that tryptophan fluores-cence of globulin free blood plasma reflects the total level of CIC and albumin. Blood plasma of 53 patients with SLE was analyzed. It was found, that plasma AOA in patients with SLE exceeds such in controls, but significant differences between patient groups were not shown. Therefore, the necessity of antioxidant therapy appears to be doubtful. It was found that the fraction of oxidized albumin in patients with SLE does not differ significantly from this parameter of healthy controls. Tryp-tophan fluorescence of globulin free plasma is reliably higher in the group with internal organ involvement, comparing to the group without it; in the group with active renal disease, comparing to the group without it, as well as in the group re-ceiving active immunosuppressive therapy including biologic therapy or therapy with high-dose steroids and cyclophos-phamide, comparing to the group receiving maintenance therapy. The correlation of this parameter with concentration of anti-DNA antibodies was shown. Therefore, it can be concluded, that tryptophan fluorescence may be used in estimation of CIC level in SLE patients’ plasma. Further investigations with larger patients group appear to be promising to obtain more reliable results. It might allow to compare levels of oxidative stress in patients with different activity and organ damage, and at various stages of treatment and follow-up to develop new diagnostic and prognostic criteria.

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