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Working out technology of monodisperse small-size range magnetite nanoparticles obtaining and evaluation of their toxicity on mice

Keywords:

L.Kh. Komissarova - Ph.D. (Biol.), Senior Research Scientist, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow
E-mail: komissarova-lkh@mail.ru
N.A. Marnautov - Post-graduate Student, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow
A.S. Tatikolov - Dr.Sc. (Chem.), Leading Research Scientist, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow
V.A. Morozov - Post-graduate Student, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow
A.B. Elfimov - Post-graduate Student, Dmitry Mendeleev University of Chemical Technology of Russia, Moscow
O.O. Vasilkov - Post-graduate Student, Dmitry Mendeleev University of Chemical Technology of Russia, Moscow


Article presents the results of working out technology of monodisperse small-size range magnetite nanoparticles obtaining by thermal decomposing of iron (III) acetylacetonate in an excess of triethylene glycol for biomedical purposes. The yield of nanoparticles was more than 90%. The size (average diameter) and structure of the particles were studied by transmission electron microscopy method. The chemical content of particles was confirmed by electronic diffraction method by comparing with magnetite standard sample. Depending on the temperature (250…290 °C) and process duration (30 min  10h) we have obtained magnetite monodisperse nanoparticles sized: 2.94±0.75 nm, 4.54±0.8 nm, 5.97±0.92 nm, 5.53±0.94nm. It has been established that the sizes of the synthesized nanoparticles depend both on the temperature and duration of reaction. On the basis of the diffraction picture of the nanoparticles obtained by SAED we can assert that the synthesized particles are the magnetite. The methods of nanoparticles magnetic separation from their suspension on Sm-Co magnet and resuspending of the particles in water were developed.
It has been established LD50=155,6 mg/kg by investigating acute toxicity of the obtained nanoparticles of 5,5±0,9 nm di-ameter by intravenously injection to mice with Spirmen-Karber method. Considering the low toxicity of the obtained mag-netite nanoparticles they can be perspective for biomedical purposes, in particular, as magnetic carriers of anti-cancer preparations and as MRT-contrasting agents of organs and tissues.

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