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Markers of oxidative stress in the body fluids of astronauts after long space flights to the ISS

DOI 10.18127/j20700997-201905-01


I.M. Larina – Dr Sc. (Med.), Professor, Chief of Proteomics laboratory, Russian Federation State Scientific Center Institute for Biomedical Problems of RAS (Moscow)
A.I. Grigoriev –
Academician of RAS, Dr. Sc. (Med.), Scientific Director of Russian Federation State scientific center Institute for Biomedical Problems of RAS (Moscow)

Оxidative stress – a potentially important secondary link in the etiopathogenesis of most dysfunctions and multiorgan disorders caused by CP in humans and experimental animals The aim of the study was to determine the presence and biological role of protein-markers of oxidative stress in the body fluids of astronauts who have completed long space flights on the ISS. Proteome analysis has identified changes associated with CP factors, levels of regulatory proteins, including participants in coagulation cascades (F5, F13A1, F13B, etc.) and innate immune system proteins (C3, CFB, SPP1, C4A, ORM1, ORM2, CD14). Studies of proteomics based on chromato-masspectrometry, biological fluids of cosmonauts after six-year space flights clearly indicate the presence of oxidative stress, as a consequence of the effects on the body of the complex adverse factors. Panoramic, semi-quantitative and quantitative methods of proteomics based on masstrorometry for the study of blood and urine reveal the proteins participating in the pro- and antioxidant system, and the direction of changes in their concentrations shows the severity oxidative stress. The findings substantiate a new approach to finding the means and methods of preventing the adverse effects of spaceflight on humans: by preventing and ceding progressive damage to cellular and tissue structures. The aim of finding the means and methods to counteract the development of oxidative stress should become the most important scientific and practical task of gravitational physiology and medicine in the stage of preparation for ultra-long-range space flights. 

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