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SPONTANEOUS REGENERATION OF ASTRAGALUS CICER L. SHOOTS FROM THE HAIRY ROOT CULTURES

Keywords:

Z.R. Vershinina, Al.H. Baimiev, A.V. Knyazev, A.V. Chemeris


Agrobacterium rhizogenes strain 15834 was used to induce hairy roots on hypocotyls of Astragalus cicer L. seedlings. After hairy roots had grown to a length of 3-4 cm, the original roots were excised, and composite plants consisting of transgenic roots on untransformed shoots were transferred into vermiculite. Two months after transferring, transformed root showed orange callus formation. Spontaneous light independent plant regeneration from callus started after 6 months of growth on 55% composite plants. Shoots regenerated had short internodes, narrow leaves and multiple branches. After 5 months of growth, plants regenerated from transformed roots showed increased biomass accumulation (600% in the roots and 200% in the shoots) in comparison to control plants.
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